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M9550059.TXT
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1995-03-04
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Document 0059
DOCN M9550059
TI Cloning and characterization of a c-myc intron binding protein (MIBP1).
DT 9505
AU Makino R; Akiyama K; Yasuda J; Mashiyama S; Honda S; Sekiya T; Hayashi
K; Oncogene Division, National Cancer Center Reseach Institute,; Tokyo,
Japan.
SO Nucleic Acids Res. 1994 Dec 25;22(25):5679-85. Unique Identifier :
AIDSLINE GENBANK/D37951
AB The cDNA for a c-myc intron 1 binding protein 1 (MIBP1) in the rat was
isolated from lambda gt11 and lambda ZAPII cDNA libraries. Sequencing of
the cDNA clones revealed a long ORF which encoded a putative protein of
2437 amino acid residues. This protein has two widely separated zinc
finger regions, each of which carries C2H2 motifs. When expressed in E.
coli as a fusion protein, part of the MIBP1 showed sequence-specific
binding to the target sequence, i.e., a 9-bp sequence in the rat c-myc
intron 1. MIBP1 is most likely the rat counterpart of human MHC binding
protein-2 (MBP-2/HIV-EP2), based on the 86% similarity in nucleotide
sequence and 93% similarity in amno acid sequence. Northern blotting
revealed a high level of MIBP1 mRNA in the brain.
DE Amino Acid Sequence Animal Base Sequence Cloning, Molecular
DNA-Binding Proteins/*GENETICS/METABOLISM Gene Expression *Genes, myc
Introns Molecular Sequence Data Rats RNA, Messenger/GENETICS
Support, Non-U.S. Gov't Transcription Factors/*GENETICS Zinc Fingers
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).